3FJE
Crystal structure of C83S mutant of Human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-14 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.414, 94.393, 108.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.730 - 2.100 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.730 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.077 | 0.248 |
Number of reflections | 21360 | |
<I/σ(I)> | 6.78 | |
Completeness [%] | 92.9 | 100 |
Redundancy | 10.1 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.0M Na formate, 0.4M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |