3FIQ
Odorant Binding Protein OBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.010, 62.070, 109.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.343 - 1.599 |
| Rwork | 0.141 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e5p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.348 | 42.010 | 1.690 |
| High resolution limit [Å] | 1.599 | 5.060 | 1.600 |
| Rmerge | 0.046 | 0.027 | 0.220 |
| Total number of observations | 4310 | 13633 | |
| Number of reflections | 37976 | ||
| <I/σ(I)> | 12.359 | 20.6 | 3.5 |
| Completeness [%] | 98.4 | 98.9 | 93 |
| Redundancy | 3.4 | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% polyethylene glycol 5000, 100mM NaF, 5% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
