3FHT
Crystal structure of human Dbp5 in complex with AMPPNP and RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00680 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.600, 79.660, 124.540 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 41.520 - 2.200 |
| R-factor | 0.1713 |
| Rwork | 0.168 |
| R-free | 0.22518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j0s |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.520 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.106 | 0.273 |
| Number of reflections | 41211 | |
| <I/σ(I)> | 10.89 | 4.54 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 4.11 | 4.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM Tris pH 7.0, 20% PEG 2000 mono-methyl-ether, 3% PEG 3350, 30mM NaF, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | tris | ||
| 10 | 1 | 1 | HOH | ||
| 2 | 1 | 1 | PEG 2000 mono-methyl-ether | ||
| 3 | 1 | 1 | PEG 3350 | ||
| 4 | 1 | 1 | NaF | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | tris | ||
| 7 | 1 | 2 | PEG 2000 mono-methyl-ether | ||
| 8 | 1 | 2 | PEG 3350 | ||
| 9 | 1 | 2 | NaF |
