3FHG
Crystal structure of Sulfolobus solfataricus 8-oxoguanine DNA glycosylase (SsOgg)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 6 |
Unit cell lengths | 110.960, 110.960, 34.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.700 - 1.900 |
R-factor | 0.226 |
Rwork | 0.207 |
R-free | 0.24800 |
Starting model (for MR) | 3fhf |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.085 | 0.536 |
Number of reflections | 37358 | 5344 |
<I/σ(I)> | 17.6 | 2.3 |
Completeness [%] | 95.9 | 100 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 50 mM sodium cacodylate, 2M (NH4)2SO4, 10mM MgSO4, (from four years old crystal tray), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |