3FH6

Crystal structure of the resting state maltose transporter from E. coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97942, 0.9794, 0.9795, 0.9494
Spacegroup name	I 2 2 2
Unit cell lengths	171.097, 209.480, 438.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.500
Rwork	0.340
R-free	0.36300
Structure solution method	MAD
Starting model (for MR)	PDB entries 1Q1B 1q1e 2r6g
RMSD bond length	0.010
RMSD bond angle	1.998
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	4.660
High resolution limit [Å]	4.500	9.690	4.500
Rmerge	0.126	0.086	0.784
Number of reflections	40160
<I/σ(I)>	11.126
Completeness [%]	87.5	93.9	69.1
Redundancy	3.4	3.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	11.5 % PEG 4000, 0.1M ADA pH 6.5, 0.1 M NaCl, 0.1 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K