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3FGM

Crystal structure of L44F/C83T/C117V/F132W mutant of Human acidic fibroblast growth factor

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]103
Detector technologyIMAGE PLATE
Collection date2008-06-01
DetectorRIGAKU RAXIS IIC
Wavelength(s)1.5418
Spacegroup nameC 2 2 21
Unit cell lengths74.385, 96.031, 108.445
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution28.880 - 1.950
R-factor0.206
Rwork0.206
R-free0.23800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1jqz
RMSD bond length0.006
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]28.8802.000
High resolution limit [Å]1.9501.950
Rmerge0.0560.194
Number of reflections26793
<I/σ(I)>8.61
Completeness [%]95.778.6
Redundancy13.28.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52984.1M Na-formate, 0.1M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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