3FGM
Crystal structure of L44F/C83T/C117V/F132W mutant of Human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.385, 96.031, 108.445 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.880 - 1.950 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.880 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.056 | 0.194 |
Number of reflections | 26793 | |
<I/σ(I)> | 8.61 | |
Completeness [%] | 95.7 | 78.6 |
Redundancy | 13.2 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4.1M Na-formate, 0.1M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |