3FG4
Crystal structure of Delta413-417:GS I805A LOX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-30 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.361, 170.382, 104.900 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 30.030 - 2.310 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fg1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.699 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.4.0066) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.030 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.124 | 0.660 |
| Number of reflections | 158438 | |
| <I/σ(I)> | 12.818 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 6-8% PEG 8000, 5% Glycerol, 0.2M CaCl2, 0.1M Imidazole acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
