3FFV

Crystal Structure Analysis of Syd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-09
Detector	MARMOSAIC 325 MM CCD MARMOSAIC 325 MM CCD
Spacegroup name	H 3
Unit cell lengths	167.600, 167.600, 41.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.900 - 2.000
R-factor	0.182
Rwork	0.182
R-free	0.23000
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.151
Number of reflections	29401
<I/σ(I)>		8.8
Completeness [%]	99.8	99.9
Redundancy	5.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	298	0.8-1.0 M sodium citrate, 0.2 M sodium chloride, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1		7	298	0.8-1.0 M sodium citrate, 0.2 M sodium chloride, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K