3FF7
Structure of NK cell receptor KLRG1 bound to E-cadherin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.502 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.120, 83.411, 56.508 |
| Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.214 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ff7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.559 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.064 | 0.058 | 0.563 |
| Number of reflections | 57098 | ||
| <I/σ(I)> | 21.542 | ||
| Completeness [%] | 98.6 | 98.8 | 88.3 |
| Redundancy | 7.3 | 9.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | PEG4000, pH 4.6, vapor diffusion, temperature 298K |
