3FEY
Crystal structure of the CBC-importin alpha complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.842, 104.505, 108.558 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.19395 |
| Rwork | 0.192 |
| R-free | 0.23803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1N54 and 1IAL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.249 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.572 |
| Number of reflections | 87239 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 83.6 | |
| Redundancy | 4.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 100 mM MES, pH 6.0, and 8% PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
