3FEM
Structure of the synthase subunit Pdx1.1 (Snz1) of PLP synthase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 154.073, 154.218, 154.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.020 |
R-factor | 0.16267 |
Rwork | 0.162 |
R-free | 0.18097 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nv1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.284 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.050 |
High resolution limit [Å] | 3.020 | 3.020 |
Rmerge | 0.075 | 0.416 |
Number of reflections | 72026 | |
<I/σ(I)> | 23.4 | 3.4 |
Completeness [%] | 99.8 | 96.2 |
Redundancy | 7.9 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 4M formate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |