3FDY
Pyranose 2-oxidase thermostable triple mutant, T169G/E542K/V546C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 101.580, 101.580, 126.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.800 - 1.550 |
R-factor | 0.18703 |
Rwork | 0.186 |
R-free | 0.21883 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2igo |
RMSD bond length | 0.022 |
RMSD bond angle | 1.981 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.300 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 1581896 | |
<I/σ(I)> | 12.8 | 2.8 |
Completeness [%] | 99.8 | 97.5 |
Redundancy | 16.4 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 10% (w/v) monomethylether PEG 2000, 0.1M Mes pH 5.2, 50mM MgCl2, 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |