3FD7
Crystal structure of Onconase C87A/C104A-ONC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.460, 51.190, 87.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.864 - 1.531 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.864 | 1.620 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.073 | 0.557 |
| Number of reflections | 31390 | |
| <I/σ(I)> | 32.52 | 5.76 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 23.35 | 18.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M ammoniumsulfate, 30% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
