3FC0
1.8 A crystal structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.609, 69.293, 73.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.760 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qdn |
RMSD bond length | 0.015 |
RMSD bond angle | 1.544 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.058 | 0.278 |
Number of reflections | 27086 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 99.3 | 93.9 |
Redundancy | 7.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 15% Tacsimate pH 7.0, 0.1M Hepes, 2% PEG 3350, Vapor diffusion, Sitting drop, temperature 298K |