3FB4
Crystal structure of adenylate kinase from Marinibacillus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.639, 46.506, 62.669 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.18275 |
| Rwork | 0.180 |
| R-free | 0.22606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1S3G |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.931 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER (for MR) |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.420 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.029 | 0.080 |
| Number of reflections | 17786 | |
| <I/σ(I)> | 24 | 9.4 |
| Completeness [%] | 98.7 | 96.9 |
| Redundancy | 2.81 | 2.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | 0.5 % LDAO,150 mM sodium chloride 1.5 M tri-Sodium citrate, 9mM p1,p5-Di(adenosine-5')pentaphosphate pentasodium salt, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
