3FAO
Crystal structure of S118A mutant 3CLSP of PRRSV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.256, 48.578, 42.918 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 44.190 - 2.010 |
| R-factor | 0.18201 |
| Rwork | 0.180 |
| R-free | 0.21466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.529 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.049 | 0.169 |
| Number of reflections | 14566 | |
| <I/σ(I)> | 47.073 | 11.8 |
| Completeness [%] | 98.9 | 96.3 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 291 | 0.1M sodium citrate pH 5.7, 0.7M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
