3F9Z

Structural Insights into Lysine Multiple Methylation by SET Domain Methyltransferases, SET8-Y245F / H4-Lys20 / AdoHcy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	200
Detector technology	CCD
Collection date	2005-03-06
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	43.990, 45.550, 94.410
Unit cell angles	89.37, 87.09, 90.50

Refinement procedure

Resolution	38.900 - 1.600
R-factor	0.21776
Rwork	0.216
R-free	0.25697
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zkk
RMSD bond length	0.016
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	90904
<I/σ(I)>	31.1	8.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% Pentaerythritol ethoxylate, 50mM Ammonium sulfate, 50mM Bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K