3F9M
Human pancreatic glucokinase in complex with glucose and activator showing a mobile flap
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-28 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.700, 81.200, 85.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.500 |
| R-factor | 0.19133 |
| Rwork | 0.190 |
| R-free | 0.22213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.388 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 73934 | |
| <I/σ(I)> | 18.9 | 3.7 |
| Completeness [%] | 93.9 | 85.4 |
| Redundancy | 3.25 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
