3F6G
Crystal structure of the regulatory domain of LiCMS in complexed with isoleucine - type II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.315, 97.943, 39.974 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.557 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.327 |
| Number of reflections | 15916 | |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris-HCl, pH8.0, 1.6M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris-HCl, pH8.0, 1.6M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
