3F59
Crystal structure of ZU5-ANK, the spectrin binding region of human erythroid ankyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.040, 204.110, 43.070 |
| Unit cell angles | 90.00, 113.14, 90.00 |
Refinement procedure
| Resolution | 36.910 - 2.000 |
| R-factor | 0.21799 |
| Rwork | 0.216 |
| R-free | 0.26428 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40191 | |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 20% PEG 4000, 0.25 M KBr, 10 mM DTT, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
