3F4Y
HIV gp41 six-helix bundle containing a mutant CHR alpha-peptide sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 37.611, 179.038, 33.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26000 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.146 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 15938 | |
| <I/σ(I)> | 28 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 1 M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
