3F4N
Crystal Structure of Pyridoxal Phosphate Biosynthetic Protein PdxJ from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-09 |
Detector | SBC-3 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.627, 114.818, 154.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.810 - 2.402 |
R-factor | 0.195 |
Rwork | 0.191 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1N5W |
RMSD bond length | 0.018 |
RMSD bond angle | 1.792 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.810 | 2.440 |
High resolution limit [Å] | 2.402 | 2.402 |
Rmerge | 0.108 | 0.600 |
Number of reflections | 75718 | |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 95.6 | 88.7 |
Redundancy | 6.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.2 M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3,350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |