3F4F
Crystal structure of dUT1p, a dUTPase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.498, 124.498, 51.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.730 - 2.000 |
| R-factor | 0.14941 |
| Rwork | 0.146 |
| R-free | 0.20713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2okd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.655 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.386 |
| Number of reflections | 53958 | |
| <I/σ(I)> | 15.1 | 4.91 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2M Na Acetate, 0.1M Tris-HCl pH 8.5, 30% PEG 4000, 10mM d-UTP, 0.015 mg/mL Trypsin. Cryoprotected with 4% Glycerol, 4% Ethylene glycol, 4% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
