3F3U
Kinase domain of cSrc in complex with inhibitor RL37 (Type III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.980890 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.090, 63.530, 73.990 |
| Unit cell angles | 78.94, 89.37, 90.06 |
Refinement procedure
| Resolution | 36.590 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.755 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.600 | |
| High resolution limit [Å] | 2.500 | 16.000 | 2.500 |
| Rmerge | 0.088 | 0.035 | 0.369 |
| Number of reflections | 25330 | 93 | 2566 |
| <I/σ(I)> | 13.15 | 31.3 | 3.6 |
| Completeness [%] | 97.2 | 93.9 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 10% PEG 4000, 0.05M sodium acetate, 4% glycerol, 0.1M MES , pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
