3F1R
Crystal structure of FGF20 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.144, 102.144, 119.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.580 - 2.500 |
| Rwork | 0.256 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.580 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 16050 | |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.2M Li2SO4, 15% Glycerol, 13% PEG 2000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
