3F12
Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-03-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.860, 87.860, 241.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.32 |
| Rwork | 0.300 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyo |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.066 | 0.333 |
| Number of reflections | 23047 | |
| <I/σ(I)> | 19.12 | 3.84 |
| Completeness [%] | 98.0 | 98.4 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.6 | 298 | 2.6 M Ammonium sulfate, 2% PEG 400 in CAPS buffer, pH 10.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
