3EZZ
Crystal Structure of human MKP-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2005-06-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 141.557, 141.557, 158.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.24200 |
Starting model (for MR) | 2g6z |
RMSD bond length | 0.009 |
RMSD bond angle | 1.370 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.071 | 0.386 |
Number of reflections | 34516 | |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 99.0 | 100 |
Redundancy | 7.4 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.2 | 291 | 0.1M CAPS pH 10.2, 0.1M NaCl, 1.7M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |