3EYW
Crystal structure of the C-terminal domain of E. coli KefC in complex with KefF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.470, 85.015, 188.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.19951 |
Rwork | 0.197 |
R-free | 0.23861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2QR2 1lss |
RMSD bond length | 0.013 |
RMSD bond angle | 1.407 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.091 | 0.391 |
Number of reflections | 39230 | |
<I/σ(I)> | 18.4 | 5.7 |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12% MPD, 10% PEG 3350, 60mM MgCl2, 100mM HEPES pH 7.0, 1mM NAD+, 1mM HALESDIE peptide, VAPOR DIFFUSION, HANGING DROP, temperature 298K |