3EX8
Complex structure of bacillus subtilis RibG reduction mechanism in riboflavin biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.078, 108.367, 187.394 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.560 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3z |
RMSD bond length | 0.008 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.650 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.062 | 0.426 |
Number of reflections | 56499 | |
<I/σ(I)> | 26.2 | 2.7 |
Completeness [%] | 97.7 | 95.4 |
Redundancy | 8.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 28 % PEG 400, 200mM CaCl2, 100mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |