3EVI
Crystal structure of the thioredoxin-fold domain of human phosducin-like protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 |
Unit cell lengths | 44.594, 44.594, 141.971 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.880 - 2.700 |
R-factor | 0.23615 |
Rwork | 0.234 |
R-free | 0.27576 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dbc |
RMSD bond length | 0.012 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.140 | 2.800 |
High resolution limit [Å] | 2.700 | 2.690 |
Rmerge | 0.175 | 0.614 |
Number of reflections | 8421 | |
<I/σ(I)> | 10.22 | 1.53 |
Completeness [%] | 93.9 | 88.6 |
Redundancy | 2.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 28%(w/v) PEG8000, 0.2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 290K |