3EVE
Crystal structure of GpppA complex of yellow fever virus methyltransferase and S-adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-09 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.422, 104.422, 51.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 66492 | |
| <I/σ(I)> | 10 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
