3EU7
Crystal Structure of a PALB2 / BRCA2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9430 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.815, 62.026, 77.975 |
| Unit cell angles | 90.00, 108.23, 90.00 |
Refinement procedure
| Resolution | 37.495 - 2.200 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w18 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.630 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.602 |
| Number of reflections | 19026 | |
| <I/σ(I)> | 16.5 | 2.9 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Na-Cacodylate pH 6.5, 0.2M magnesium acetate, 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
