2W18

Crystal structure of the C-terminal WD40 domain of human PALB2

Summary for 2W18

• Entry DOI: 10.2210/pdb2w18/pdb
• Descriptor: PARTNER AND LOCALIZER OF BRCA2, GLYCEROL (3 entities in total)
• Functional Keywords: fanconi anemia, homologous recomination, polymorphism, phosphoprotein, beta-propeller, wd40, fanc-n, nucleus, wd repeat, coiled coil, nuclear protein
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Nucleus: Q86YC2
• Total number of polymer chains: 1
• Total formula weight: 39624.26

Authors

Oliver, A.W.,Pearl, L.H. (deposition date: 2008-10-15, release date: 2009-07-28, Last modification date: 2024-05-08)

Primary citation

Oliver, A.W.,Swift, S.,Lord, C.J.,Ashworth, A.,Pearl, L.H.
Structural Basis for Recruitment of Brca2 by Palb2
Embo Rep., 10:990-, 2009

PubMed Abstract: The breast cancer 2, early onset protein (BRCA2) is central to the repair of DNA damage by homologous recombination. BRCA2 recruits the recombinase RAD51 to sites of damage, regulates its assembly into nucleoprotein filaments and thereby promotes homologous recombination. Localization of BRCA2 to nuclear foci requires its association with the partner and localizer of BRCA2 (PALB2), mutations in which are associated with cancer predisposition, as well as subtype N of Fanconi anaemia. We have determined the structure of the PALB2 carboxy-terminal beta-propeller domain in complex with a BRCA2 peptide. The structure shows the molecular determinants of this important protein-protein interaction and explains the effects of both cancer-associated truncating mutants in PALB2 and missense mutations in the amino-terminal region of BRCA2.

PubMed: 19609323
DOI: 10.1038/EMBOR.2009.126

Experimental method

X-RAY DIFFRACTION (1.9 Å)