3EU5
Crystal structure of FTase(ALPHA-subunit; BETA-subunit DELTA C10) in complex with BiotinGPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0007 |
| Spacegroup name | P 61 |
| Unit cell lengths | 175.065, 175.065, 70.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.960 - 2.800 |
| R-factor | 0.1553 |
| Rwork | 0.153 |
| R-free | 0.20692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ft1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.784 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 30615 | |
| <I/σ(I)> | 20.5 | 5.9 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% (w/v) PEG 4000, 0.2M MgCl2, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
