3ESL
Crystal structure of the conserved N-terminal domain of the mitotic checkpoint component BUB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.304, 59.784, 71.299 |
| Unit cell angles | 90.00, 97.96, 90.00 |
Refinement procedure
| Resolution | 34.920 - 1.740 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.075 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.039 | 0.249 |
| Number of reflections | 54924 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.6 | 291 | LITHIUM SALT, pH 9.60, VAPOR DIFFUSION, temperature 291K |
