3EPS
The crystal structure of isocitrate dehydrogenase kinase/phosphatase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.919 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 124.595, 124.595, 267.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 | |
| Number of reflections | 45600 | |
| <I/σ(I)> | 305.7 | 1.4 |
| Completeness [%] | 86.5 | 96.6 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12% PEG8000, 0.2M magnesium chloride, 0.1M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
