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3EP3

Human AdoMetDC D174N mutant with no putrescine bound

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE A1
Synchrotron siteCHESS
BeamlineA1
Temperature [K]100
Detector technologyCCD
Collection date2006-02-01
DetectorADSC QUANTUM 210
Wavelength(s)0.9764
Spacegroup nameC 1 2 1
Unit cell lengths90.598, 55.240, 74.224
Unit cell angles90.00, 109.64, 90.00
Refinement procedure
Resolution34.950 - 1.840
R-factor0.243
Rwork0.243
R-free0.28200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1i7b
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.910
High resolution limit [Å]1.8401.840
Rmerge0.0640.390
Number of reflections26141
<I/σ(I)>15.8692.2
Completeness [%]92.173.8
Redundancy3.22.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP829516% PEG 8000, 100 mM Tris, 10 mM DTT, pH 8.0, vapor diffusion, hanging drop, temperature 295K

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