3EOT
Crystal structure of LAC031, an engineered anti-VLA1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.163, 132.425, 41.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.900 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.27000 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.060 | 0.041 | 0.218 |
Number of reflections | 36217 | ||
<I/σ(I)> | 35.365 | ||
Completeness [%] | 96.3 | 99.4 | 79 |
Redundancy | 6.7 | 6.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-20% Peg 3350, 100 mM Na Cacodylate, 200 mM MgCl2 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |