3EOB
Crystal structure the Fab fragment of Efalizumab in complex with LFA-1 I domain, Form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-27 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 111.096, 111.096, 470.726 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.600 |
R-factor | 0.269 |
Rwork | 0.267 |
R-free | 0.33300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.203 | 0.622 |
Number of reflections | 20645 | |
<I/σ(I)> | 7.5 | 2 |
Completeness [%] | 97.8 | 98.9 |
Redundancy | 5.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 0.1M sodium cacodylate, 0.2M zinc acetate, 11% w/v PEG 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |