3EO2
Crystal structure of the RhoGEF domain of human neuroepithelial cell-transforming gene 1 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 64 |
Unit cell lengths | 95.629, 95.629, 62.887 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z0q |
RMSD bond length | 0.014 |
RMSD bond angle | 1.123 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.095 | 0.047 | 0.526 |
Number of reflections | 9673 | ||
<I/σ(I)> | 12.9 | ||
Completeness [%] | 95.1 | 98.4 | 65.3 |
Redundancy | 8.8 | 9.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 1.0M sodium citrate, 0.1M sodium cacodylate, 5% ethylene glycol, crystallized in the presence of 1:100 (w/w) subtilisin, pH 4.8, vapor diffusion, hanging drop |