3EO1

Structure of the Fab Fragment of GC-1008 in Complex with Transforming Growth Factor-Beta 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	CCD
Collection date	2006-09-24
Detector	MARRESEARCH
Wavelength(s)	1.070645
Spacegroup name	P 21 21 21
Unit cell lengths	149.040, 149.130, 149.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 3.100
R-factor	0.255
Rwork	0.255
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eo0 1tgj
RMSD bond length	0.006
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.300
High resolution limit [Å]	3.100	3.100
Rmerge	0.092	0.367
Number of reflections	60296
<I/σ(I)>	20.8	3.8
Completeness [%]	97.9	96.4
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	23% tert.-butanol, 0.1M sodium citrate, 0.2M phenoxyacetic acid, pH6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K