3EJX
Crystal structure of diaminopimelate epimerase from Arabidopsis thaliana in complex with LL-AziDAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115879 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.540, 136.439, 102.570 |
| Unit cell angles | 90.00, 119.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gke |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.307 |
| Number of reflections | 175374 | |
| <I/σ(I)> | 12.2 | 2.6 |
| Completeness [%] | 98.0 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 60% v/v Tacsimate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
