3EJL
Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.435, 94.642, 131.988 |
| Unit cell angles | 90.00, 122.35, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.232 |
| R-free | 0.25700 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.515 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.085 | 0.457 |
| Number of reflections | 27289 | |
| <I/σ(I)> | 22.408 | |
| Completeness [%] | 78.5 | 26.4 |
| Redundancy | 2.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris,PH8.0, 30mM ammonium sulfate, 24%PEG4000, 20mM BME, pH 8.0 , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
