3EID
CDK2/CyclinA complexed with a pyrazolopyridazine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 184.506, 184.506, 213.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 3.150 |
| R-factor | 0.20702 |
| Rwork | 0.204 |
| R-free | 0.26071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 31913 | |
| <I/σ(I)> | 21 | 3.8 |
| Completeness [%] | 89.2 | 91 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 1.5 M sodium acetate, 100 mM sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
