3EI2
Structure of hsDDB1-drDDB2 bound to a 16 bp abasic site containing DNA-duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.210, 123.480, 159.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.111 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.590 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.051 | 0.357 |
Number of reflections | 69431 | 7339 |
<I/σ(I)> | 27.4 | 6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 100mM Ca-Acetate, 100mM MES pH 5.7, 12-14% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ca-Acetate | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | PEG 400 | ||
4 | 1 | 2 | Ca-Acetate | ||
5 | 1 | 2 | MES | ||
6 | 1 | 2 | PEG 400 |