3EGV
Ribosomal protein L11 methyltransferase (PrmA) in complex with trimethylated ribosomal protein L11
Replaces: 3CJUExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 62 |
Unit cell lengths | 134.547, 134.547, 48.944 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.18416 |
Rwork | 0.183 |
R-free | 0.21332 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 2NXC 2nxn |
RMSD bond length | 0.015 |
RMSD bond angle | 1.503 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.056 | 0.334 |
Number of reflections | 48051 | |
<I/σ(I)> | 12.2 | 2 |
Completeness [%] | 97.2 | 93.8 |
Redundancy | 1.7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 6.5 | 277 | 160 mM calcium acetate hydrate, 80 mM sodium cacodylate, 14.4% w/v PEG8000, 20% v/v glycerol, 4mM AdoMet, pH 6.5, microbatch technique under oil, temperature 277.0K |