3EGD
Crystal structure of the mammalian COPII-coat protein Sec23a/24a complexed with the SNARE protein Sec22 and bound to the transport signal sequence of vesicular stomatitis virus glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.750, 97.630, 129.120 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.206 |
| R-free | 0.27400 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.309 |
| Number of reflections | 46345 | |
| <I/σ(I)> | 15.5 | 3 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 10% (w/v) PEG 4000, 0.1 M NaCl, 0.5 M sodium acetate, 50 mM Tris buffer, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
