3EFH
Crystal structure of human phosphoribosyl pyrophosphate synthetase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | H 3 |
Unit cell lengths | 169.180, 169.180, 61.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.21649 |
Rwork | 0.216 |
R-free | 0.23133 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hcr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.295 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.065 | 0.345 |
Number of reflections | 18518 | |
<I/σ(I)> | 8.5 | 1.8 |
Completeness [%] | 91.2 | 88.5 |
Redundancy | 1.4 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 295 | 2.1M ammonium sulfate, 0.1M sodium acetate, 10% (v/v) glycerol, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |