3EF5
Structure of the RNA pyrophosphohydrolase BdRppH in complex with dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-16 |
Detector | adsc Q210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.446, 68.448, 92.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.370 - 2.600 |
R-factor | 0.243 |
Rwork | 0.240 |
R-free | 0.29700 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.246 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.370 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.049 | 0.027 | 0.691 |
Number of reflections | 15730 | ||
<I/σ(I)> | 32.267 | ||
Completeness [%] | 89.2 | 99.4 | 40.7 |
Redundancy | 6.6 | 6.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 298 | PEG 4000, Na acetate, pH 7, hanging drop, temperature 298K |