3EEU

Structure of the RNA pyrophosphohydrolase BdRppH in complex with Holmium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-26
Detector	RIGAKU RAXIS4
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	70.212, 70.212, 99.915
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.400 - 2.000
R-factor	0.218
Rwork	0.216
R-free	0.25600
RMSD bond length	0.010
RMSD bond angle	1.361
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.039	0.021	0.775
Number of reflections	19802
<I/σ(I)>	43.438
Completeness [%]	99.9	99	100
Redundancy	5.4	5.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	4	298	PEG 4000, Na acetate, hanging drop, temperature 298K